Direct Bioautography For Antifungal Activity Measurement “case Of Bayoud Disease”

Fusarium oxysporum f. sp. albedinis (Foa) is the causal agent of a lethal disease of date palm “Phoenix dactylifera L.” called Bayoud. The antifungal test against Foa was evaluated using direct bioautography for extracts from four medicinal and/or poisonous plants (Acacia raddiana, Asteriscus graveolens (Forsk.), Citrullus colocynhis (L.) Schrad, Pergularia tomentosa). The choice of extracts was based on activity against Foa using disc diffusion techniques and relative virulence. The most effective extracts (detected inhibition and relative virulence decreased below 50%) were chosen for direct bioautography test. Direct bioautography has a great importance based on effectiveness. Extracts from Acacia raddiana showed no effect on chromatograms. The best results were represented by ethyl acetate extract of Citrullus colocynthis fruits (7.75  1.06 mm) and ethyle acetate extract of Asteriscus graveolens stems (7.001.41 mm). The efficiency of some species especially Citrullus colocynthis indicates the presence of highly sensitive targets in foa, which can be exploited for the development of efficient treatment against Bayoud.